Researchers at the University of Wisconsin-Madison have identified a gene that keeps grasses from entering their flowering cycle until the season is right, a discovery that may help plant breeders and engineers get more from food and energy crops.
Many plants have evolved the ability to wait for snow to melt before investing precious resources in blooms.
Flowering as the warmer, longer days of spring arrive — and the risk of a damaging frost recedes — requires a process called vernalisation, a news release from the university (HERE)explains.
This is a process in which flowering is blocked until the plant senses a sufficient cold spell.
Some varieties of wheat are among the plants that prefer to get established in the autumn but avoid flowering before it gets cold, says Rick Amasino, a UW-Madison professor of biochemistry and genetics.
By becoming established in the autumn, these plants can take full advantage of the window of the growing season when it opens in the spring.
Much has been done to identify genes involved in flowering, including one in grasses called VRN1 that helps get the vernalisation ball rolling by spurring groups of other genes into action. But just what keeps VRN1 in check, so flowering does not occur in the fall or in a winter warm spell, was unclear until Amasino, postdoctoral researcher Daniel Woods and others began putting a small Mediterranean grass called Brachypodium, or false purple brome, through false cold seasons in lab refrigerators.
“Getting at the genetics underlying complex processes is difficult in many crop species, so we’ve used a small plant with a compact genome as a model to get at the molecular underpinnings of how a vernalisation requirement is established, says Woods, first author on the study published in the journal Proceedings of the National Academy of Sciences.
“What we found is a gene that represses the VRN1 gene prior to winter.”
Comparing the DNA of Brachypodium plants that abide by the typical cold-to-warm transition before flowering, to DNA from mutant versions of the grass that flower without the typical wait, pointed the researchers to a gene they are calling RVR1 (for its role in repressing VRN1).
They suspect RVR1 serves the same purpose in other temperate grasses that require vernalisation, a group that plays an outsized role in our lives.
“Grasses — including corn, wheat, oats, rye and barley — provide more than 80 percent of our caloric intake worldwide,” Woods says. “Rice alone in some countries is as high as 70 percent of the calories. But if you combine things we eat directly as well as animals we depend on that also eat grass, it is clear that grasses make the world go around.”
While the newly identified gene will likely be of interest to breeders of cereal grains, all those grass calories fuel more than bodies, says Amasino, whose work is supported by the National Science Foundation and the US Department of Energy’s Great Lakes Bioenergy Research Center (GLBRC).
Switchgrass, which holds interest as feedstock for production of biofuels such as ethanol, doesn’t bother with vernalisation. It grows slowly and flowers in the early summer months in the upper Midwest.
“We think there’s a good chance that taking the RVR1 gene from Brachypodium and putting it in switchgrass will delay switchgrass flowering,” Amasino says. “Delaying switchgrass flowering to various extents may affect and improve yield.”
Amasino’s lab will partner with GLBRC researchers on that work, and will continue to study different types of Brachypodium adapted to winters spanning a range of temperatures and lengths.
“How did one variety evolve a system tweaked to require 16 weeks of cold? How did another one develop for just two weeks of cold? What’s the genetic difference between the requirement for a short winter versus a long winter?” Amasino says.
Grasses are important crops, and this model for studying flowering can tell scientists a lot about how they work.